Phenotypic Switching and β-N-Acetylhexosaminidase Activity of the Pathogenic YeastTrichosporon asahii
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Purification and Characterization of β-N-Acetylhexosaminidase from Rice Seeds
N-Acetyl-β-D-hexosaminidase (β-HexNAc’ase) (EC 3.2.1.52) was purified from rice seeds (Oryza sativa L. var. Dongjin) using ammonium sulfate (80%) precipitation, Sephadex G150, CM-Sephadex, and DEAE-Sephadex chromatography, sequentially. The activities were separated into 7 fractions (F1F7) by CM-Sephadex chromatography. Among them, F6 was further purified to homogeneity with a 13.0% yield and 1...
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Serum N-acetyl-beta-D-glucosaminidase (NAG; EC 3.2.1.30) is a hexosaminidase and may be a predictor of vascular injury, e.g., in infant respiratory distress syndrome, pneumonia, broncho-pulmonary dysplasia and necrotizing enterocolitis. To estimate the new diagnostic prospects we have modified our urinary NAG assay. In this sensitive colorimetric micro-assay, VRA-GlcNAc was used as a substrate....
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A variety of fungi produce the hydrolytic enzyme beta-N-acetylhexosaminidase (HexNAcase), which can be readily detected in assays by using p-nitrophenyl-N-acetyl-beta-D-glucosaminide as a substrate. In the present study we developed a microtiter plate-based HexNAcase assay for distinguishing Candida albicans and Candida dubliniensis strains from other yeast species. HexNAcase activity was detec...
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Three and four N-acetylhexosaminidase components, from ram testis and epididymis respectively, have been separated by ion-exchange chromatography on DEAE-cellulose. Although they all have the same molecular weight (approx. 140000) and very similar catalytic properties towards the synthetic substrates, 4-methylumbelliferyl N-acetyl-beta-glucosaminide and N-acetyl-beta-galactosaminide, isoelectri...
متن کاملPurification and characterization of isoforms of β-N- acetylhexosaminidase from mungbean seedlings
Three isoforms of β-N-acetylhexosaminidase (β-NAHA), named β-NAHAs I, II and III, were isolated from six-day-old etiolated mungbean (Vigna radiata) seedlings. β-NAHA I was purified to apparent homogeneity by a procedure involving Con A-Sepharose chromatography, chromatofocusing, and gel filtration. β-NAHAs II and III were highly purified. β-NAHAs I, II and III had molecular masses of 135,127 an...
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ژورنال
عنوان ژورنال: Microbiology and Immunology
سال: 2004
ISSN: 0385-5600
DOI: 10.1111/j.1348-0421.2004.tb03519.x